Figure 5.
IPS-1 signaling mediates RSV-induced expression of cytokines, chemokines and PRRs in the antigen presenting cells of the lung. mRNA expression of IFNα, IFNβ, IL-1β, IL-6, CXCL1 and CCL2 in primary cultures of alveolar epithelial cells (AEC; A), alveolar macrophages (MΦ; B), interstitial macrophages (C), pulmonary CD11b+ (D) and CD103+ dendritic cells (E), isolated from WT versus IPS-1 KO lungs. F, Overview of Mda5, RIG-I, TLR3 and TLR7 expression in different cell types of IPS-1 KO lungs, expressed as % of the mRNA levels observed in WT cells. All expression data are determined by RT-PCR 12 hours post infection with RSV (expressed as fold increase over controls; mean ± SEM; n = 4–5).