Figure 2. SCRG1 stimulates ERK, JNK, and PI3K pathways in hMSCs.

(a) UE7T-13 cells were serum-starved overnight and stimulated with 500 ng/mL rhSCRG1. (b) AdSCRG1-FLAG–transfected UE7T-13 cells were cultured with serum-reduced DMEM for 1 week. (c) UE7T-13 cells cultured in osteogenic differentiation medium (ODM) for 1 week were serum-starved overnight and stimulated with 500 ng/mL rhSCRG1. Cells in (a–c) were washed twice with ice-cold PBS and lysed in RIPA buffer. Samples were separated by SDS-PAGE and analyzed by western blotting. Although cropped blots were used, the gels were run under the same experimental conditions.