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. 2014 Jan 13;4:3652. doi: 10.1038/srep03652

Figure 6. SCRG1/BST1 preserves self-renewal potential and the expression of stem cell markers in hMSCs even after long-term culture.

Figure 6

(a–c) Primary cultured hMSCs (passage number #5) were subcultured ten times in the presence (passage number #15, +rhSCRG1) or absence (passage number #15, −rhSCRG1) of 500 ng/mL rhSCRG1. These primary hMSCs under long-term culture conditions were used for the experiments in a–c. (a) The cells were cultured in growth medium on 96-well culture plates for 5 days. After incubation, proliferation was evaluated by WST-1 assay. (b) Expression of cell surface CD271/LNGFR (left column), CD90/THY-1 (middle column), and CD106/VCAM1 (right column) was analyzed by flow cytometry with FITC- or PE-conjugated specific antibodies. Specific antibody (red) and isotype control IgG (blue) are shown. (c) mRNA expression of POU5F1 (left panel) and NANOG (right panel) were analyzed by qRT-PCR and normalized to GAPDH; results are expressed as fold-increase or decrease relative to the control. In a–d, data are presented as mean ± SD. *p < 0.05 was considered significant.