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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Acta Biomater. 2013 Nov 4;10(2):604–612. doi: 10.1016/j.actbio.2013.10.033

Fig. 5.

Fig. 5

Morphology and flow cytometry data for hESC-derived CPCs seeded onto hydrogels on day 6. (A) Differentiation was initiated in H9 cells on TCPS on day 0, and Nkx2.5/Isl1+ CPCs were singularized and replated onto hydrogels or TCPS on day 6. Representative images from day 13 show that H9-derived CPCs self-aggregated into tissue-like structures on all hydrogel stiffnesses, but not TCPS. For comparison, an unsplit well on day 13 is shown. Scale bar = 100 µm. (B) Flow cytometric analysis of replated CPCs on day 15. % cTnT+ did not significantly differ among hydrogel stiffnesses or TCPS; however, the percentage of cTnT+ cells on the 50 kPa hydrogel was significantly lower than the unsplit control (p < 0.05). 3 wells of each hydrogel stiffness, 3 wells of TCPS, and 2 unsplit wells were measured.