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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 Dec 15;21(1):103–109. doi: 10.1038/nsmb.2737

Figure 7. Effect of Mec1 and Tel1 kinases on spreading in cis and trans from a DSB induced near CEN2.

Figure 7

An HO-induced DSB was created by galactose-mediated induction at a site 13.5 kb from CEN2. Increases in γ-H2AX were measured at the times indicated for a site 17.7 kb distal to the DSB on the same chromosome (a), 9.9 kb from CEN6 (b) and overlapping CEN14 (c). Fold increase is calculated by normalizing IP value to input and then to 0hr. Note that in tel1Δ strains there is a lower level of spreading in both cis and trans, whereas the absence of MEC1 specifically affects spreading in trans. Error bars represent the range from two independent experiments.