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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: J Immunol. 2013 Nov 11;191(12):6200–6207. doi: 10.4049/jimmunol.1300744

FIGURE 1.

FIGURE 1

Genes regulated in common by IL-2 and NPM/ALK. The ALK-TCL derived, IL-2 dependent Sez-4 cell line was cultured in medium deprived of IL-2 for 18h and subsequently re-stimulated with IL-2 (200 units) for 4h. The ALK+TCL derived Sudhl-1 cell line was treated with an ALK-specific inhibitor (175nM) for 6h. Cells were harvested and total RNA reverse transcribed to cDNA and hybrydized to Affymetrix HG-U133 Plus 2 arrays containing DNA probes for >47,000 genes. The results were analyzed using GeneSpring and Partek Genomics Suite software. Blue circles: genes modulated in the Sez-4 cell line in response to IL-2 (cut off: Fold change >1.5(A), no fold change used as selection criteria (B) and FDR>0.05 (A and B). Pink circles: genes modulated in the Sudhl-1 cell line in response to the ALK inhibitor (cut off: Fold change >1.5(A), no fold change used as selection criteria (B) and FDR>0.05 (A and B)). Areas where two circles overlap represents the commonly regulated 1302 and 5909 genes between both cell lines. The experiments were performed in triplicates (N=3). The entire microarray data sets are available in the NIH database GEO at www.ncbi.nlm.nih.gov/geo under the accession numbers GSE8685 and GSE50803.