Fig. 4.

Histology of the rhMOGF/IFA model in cynomolgus monkeys. a–f Lesions in the brain of cynomolgus monkey C1. a Overview with HE stain (magnification x0.8). The rectangle indicates features of pallor and inflammation. b Overview with LFB/PAS stain (x0.8). The rectangle indicates a demyelinated area. c Macrophagic perivascular inflammation (HE stain, x100). Insert: HE stain, x400. d Macrophages containing intracytoplasmic LFB + myelin degradation products (x400). Insert: PLP stain, macrophages. e, f A perivascular cuff is shown (both x400). e CD3 stain. f CD20 stain. g–k Lesions in the brain of cynomolgus monkey C2. g Overview with HE stain (x0.8). The rectangle indicates features of pallor and inflammation. h Overview with LFB/PAS stain (x0.8). The rectangle indicates a demyelinated area. i Macrophagic infiltration (HE stain x400). Note the vacuolated cytoplasm of the macrophages. Insert: Macrophages containing PAS + and LFB + myelin degradation products. j Overview of cerebellar white matter (LFB/PAS stain, x10). Demyelinated area is outlined in black. Insert: Pale luxol staining associated with remyelination. k Vacuolated macrophages (HE stain, x100). Insert: PLP immunostaining. Absence of myelin. l Brain of cynomolgus monkey C4. Macrophagic and neutrophilic dense tissue infiltrate (HE stain, x100). Insert: MRP8 immunostaining