Fig. 1.

FBMI euthanasia design and testing. (a) FBMI euthanasia was performed in a Muromachi 10 kW Microwave fixation system. (b) Mice are anesthetized and placed in an animal holder. The holder includes a water compartment filled with ionic buffer solution that aligns the mouse brain in the center of the waveguide. (c) An ASTIM phantom was manufactured in order to develop standard operating microwave irradiation protocols that enable adequate heat distribution in the brain without boiling. (d) The ASTIM phantoms were used to test the effect of irradiation time and buffer solution for the rodents