Fig. 1.

miR-331-3p is down regulated in GBM cell lines compared to normal brain and inhibits cell proliferation and clonogenicity. a TaqMan RT-qPCR analysis of miR-331-3p expression in U-87 MG and U-251 MG cells relative to normal brain RNA. b Cell titre assay of U-251 MG cell proliferation following transient transfection with either miR-331-3p or negative control miRNA (miR-NC). c Clonogenicity assay of U-251 MG cells 12 days following transient transfection with either miR-331-3p or miR-NC. Error bars represent standard deviations; *p < 0.05, **p < 0.01