Fig. 4.

Effect of 2.5 mM of eATP on anchorage-dependent and anchorage-independent hepatoma cells. BEL7402 hepatoma cells were cultured in six-well plates without or with poly-HEMA for 24 h as anchorage-dependent and anchorage-independent hepatoma models. Then, cells were treated with 2.5 mM of eATP and harvested for Western blot analysis at 0, 1, 3, 6, 9, and 12 h after eATP treatment. a Western blot assay of caspase 3 activation, LC3-II procession, and AMPK and mTOR pathway activation in the anchorage-dependent hepatoma model. b–e Densitometric analysis of Western blot bands was calculated using Image J software and normalized to the internal control (β-actin). f Western blot analysis of activation of caspase 3, LC3-II, and phosphorylation of AMPK and mTOR pathways in the anchorage-independent hepatoma model. g–j Densitometric analysis of Western blot bands was calculated using Image J software and normalized to β-actin. Presented figures are representative data from three independent experiments. **P < 0.01