Fig. 5.

Treatment of anchorage-dependent and anchorage-independent hepatoma cells with BzATP. a Anchorage-dependent and anchorage-independent BEL7402 cells were treated with 1 or 2.5 mM of BzATP and cultured for 24 h. Activation of caspase 3, LC3-II, and phosphorylation of AMPK and mTOR pathways were detected by Western blot. b, d, f, h, j Caspase 3 (b), LC3-II (d), p-AMPK (f), p-mTOR (h), and p-S6K1 (j) in anchorage-dependent cells were quantified by densitometric analysis and normalized to β-actin. c, e, g, i, k Caspase 3 (c), LC3-II (e), p-AMPK (g), p-mTOR (i), and p-S6K1 (k) in anchorage-independent cells were quantified by densitometric analysis and normalized to β-actin. Presented figures are representative data from three independent experiments. *P < 0.05; **P < 0.01. NS not statistically significant