Fig. 7.

Treatment of anchorage-dependent and anchorage-independent hepatoma cells with apyrase and eATP. a Anchorage-dependent and anchorage-independent BEL7402 cells were treated with 2.5 mM of eATP in the absence or presence of apyrase (1 U/mL). Cell viabilities were measured by CCK-8 assay after incubation for 24 h. b Anchorage-dependent and anchorage-independent BEL7402 cells were pretreated with apyrase for 0.5 h, followed by 2.5 mM of eATP treatment for 24 h before harvested for Western blot analysis. c–l Caspase 3 (c, d), LC3-II (e, f), p-AMPKα (g, h), p-mTOR (i, j), and p-S6K1 (k, l) in two types of cells were quantified by densitometric analysis and normalized to β-actin (c, e, g, i, and k for anchorage-dependent model and d, f, h, j, and l for anchorage-independent model). Presented figures are representative data from three independent experiments. *P < 0.05; **P < 0.01