Fig. 8.

Treatment of anchorage-dependent and anchorage-independent hepatoma cells with KN-62 and eATP. Anchorage-dependent and anchorage-independent BEL7402 cells were preincubated with KN-62 (1 μM) and then treated with 2.5 mM of eATP for 24 h. a Cell viabilities were tested by CCK-8 analysis. b Cells were harvested for Western blot analysis. c–l Caspase 3 (c, d), LC3-II (e, f), p-AMPKα (g, h), p-mTOR (i, j), and p-S6K1 (k, l) bands were quantified densitometrically by using Image J software. Caspase 3, LC3-II, p-AMPKα, p-mTOR, and p-S6K1 in two types of cells were quantified by densitometric analysis and normalized to β-actin (c, e, g, i, and k for anchorage-dependent model and d, f, h, j, and l for anchorage-independent model). Presented figures are representative data from three independent experiments. *P < 0.05; **P < 0.01