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. Author manuscript; available in PMC: 2014 Nov 27.
Published in final edited form as: J Med Chem. 2013 Nov 15;56(22):9265–9274. doi: 10.1021/jm401340p

Figure 1.

Figure 1

Binding affinity of 16d to wt M2TM as determined by solution NMR drug titration. Normalized peak volume of the W41 Hε1 in the drug-bound form was plotted as a function of 16d concentration. The fitting yields a stoichiometry ratio of 1.37 ± 0.28 drug/tetramer with a Kd of 40 ± 24 μM (curve shown in blue). In comparison, theoretical curve fittings with fixed stoichiometry (N=1) and Kd of 4 μM, 100 μM and 400 μM are shown in red, green, and pink, respectively. Details about curve fitting can be found in the Supporting Information.