Table 3.
Activity of guanidine compounds in influenza virus yield assay.
| Compound | Activitya (μM) against A/HK/7/87
|
Cytotoxicity at 24 hr
|
|
|---|---|---|---|
| EC90 (μM) | EC99 (μM) | MCC (μM)b | |
| 16a | 0.46 | 0.79 | >50 |
| 16b | 20 | ≥38 | >50 |
| 14d | 0.85 | >10 | >50 |
| 16d | 0.37 | >2 | ≥10 |
| Amt | 0.22 | 1.1 | ≥500 |
| Rimantadine | 0.10 | >4 | ≥500 |
| Ribavirin | 4.6 | 11 | >100 |
a
Antiviral activity was determined by a qRT-PCR based virus yield assay to quantify the virus in the supernatant at 24 post infection.35 EC90 and EC99: concentrations causing 1 log10 and 2 log10 reduction in virus yield, respectively.
b
MCC: minimum cytotoxic concentration, or concentration producing minimal alterations in cell morphology after 24 hr incubation with compound.