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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Cell Biochem. 2014 Jan;115(1):179–188. doi: 10.1002/jcb.24648

Figure 6. Effects of ROS on PTH1R expression.

Figure 6

(A) Realtime RT-qPCR analysis of PTH1R expression in MC3T3-E1 cells treated with xanthine (25 μM) and the indicated concentrations of xanthine oxidase. *p < 0.05 vs. control. (B) Immunofluorescence images of MC3T3-E1 cells treated with vehicle or X/XO (25 μM/25 mU/mL) using anti-PTH1R antibody. Magnification bar − 200 μm. (C) Realtime RT-qPCR analysis of PTH1R expression in MC3T3-E1 cells treated with vehicle, isoPGE2 (25 μM) or X/XO (25 μM/25 mU/mL). *p < 0.05 vs. vehicle control.