Skip to main content
. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Cell Biochem. 2014 Jan;115(1):179–188. doi: 10.1002/jcb.24648

Figure 8. Effects of antioxidants on the inhibitory effects of bioactive inflammatory lipids.

Figure 8

(A) Alkaline phosphatase activity of MC3T3-E1 cells that were pretreated with vehicle or Trolox at the indicated concentrations for 1 hr followed by co-treatment with Trolox and isoPGE2 for 6 days. (B) Immunofluorescence images of MC3T3-E1 cells that were pretreated with vehicle or Trolox (400 μM) for 1 hr, followed by co-treatment with vehicle, ox-PAPC (10 μg/ml) and/or Trolox for 6 days. Magnification bar − 200 μm. (C) Quantitative analysis of PTH1R immunofluorescence shown in (B). (D) The levels of PTH1R in MC3T3-E1 cells that were pretreated with vehicle or Trolox (400 μM) for 1 hr, followed by cotreatment with vehicle, isoPGE2 (30 μM) and/or Trolox for 6 days. Fluorescence images were quantified using Metamorph image analysis. *p < 0.05 vs. vehicle, #p < 0.05 vs. isoPGE2 or ox-PAPC.