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. 2013 Dec;20(12):1827–1835. doi: 10.1128/CVI.00519-13

Fig 1.

Fig 1

Comparison of IFN-γ responses to liquid and lyophilized antigen preparations from control (n = 5) as well as M. bovis Ravenel- (n = 5) and M. bovis 95-1315-infected (n = 5) animals. (a) Response kinetics: PPDb (Lelystad) minus no stimulation. For control animals, the responses to liquid and lyophilized antigens were equivalent; thus, the lines on the graphs are overlapping. Responses to PPDb detected prior to challenge in all groups (including controls) were likely due to cross-reactive responses elicited by nontuberculous Mycobacteria spp. (b) Liquid and lyophilized antigen preparations pooled over time. Responses to pokeweed mitogen (PWM) are included as a positive controls for cell viability. (c) Response kinetics: PPDb minus PPDa (Lelystad PPDs).