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. 2013 Dec;20(12):1817–1826. doi: 10.1128/CVI.00596-13

Fig 1.

Fig 1

SDS-PAGE analysis of pilot scale factor Xa cleavage of MBP fusion proteins. (A) MBP-MAP0435c; (B) MBP-MAP1017c; (C) MBP-MAP1846. Lanes 1, molecular mass markers (PageRuler Plus prestained protein ladder); lanes 2, MBP-LacZ (54 kDa); lanes 3, MBP-M. avium subsp. paratuberculosis fusion protein before cleavage; lanes 4 to 10, MBP-M. avium subsp. paratuberculosis fusion proteins cleaved at different time points, i.e., 16 h (lanes 4), 18 h (lanes 5), 20 h (lanes 6), 25 h (lanes 7), 30 h (lanes 8), 35 h (lanes 9), and 40 h (lanes 10).