SDS-PAGE of factor Xa-cleaved M. avium subsp. paratuberculosis proteins. Lanes 1, molecular mass markers (PageRuler Plus prestained protein ladder); lanes 2, MBP-LacZ; lanes 3, 5, 7, and 9, MBP-M. avium subsp. paratuberculosis fusion proteins; lanes 4, 6, 8, and 10, factor Xa-cleaved MBP and M. avium subsp. paratuberculosis proteins.