Table 2.
Expected molecular masses of MBP fusion and cleaved proteins
| Cluster | Protein | CTa (h) | Molecular mass (kDa) of: |
|
|---|---|---|---|---|
| Fusion proteinb | M. avium subsp. paratuberculosis protein | |||
| Amino acid metabolism | MAP1297 | 40 | 67.8 | 25.3 |
| MAP1846c | 40 | 73 | 30.5 | |
| Antioxidant enzymes | MAP1588c | 20 | 61.3 | 18.8 |
| MAP1589c | 16 | 64.1 | 21.6 | |
| MAP1653 | 16 | 58.9 | 16.4 | |
| ATP biosynthesis | MAP3393c | 20 | 60 | 17.5 |
| Cell division | MAP0068 | 40 | 60 | 17.5 |
| Cellular processes | MAP1560 | 16 | 57.7 | 15.2 |
| MAP1885c | 16 | 60.9 | 18.4 | |
| MAP2411 | 16 | 58 | 15.5 | |
| MAP2705c | 20 | 56.4 | 13.9 | |
| MAP3538 | 16 | 58.4 | 15.9 | |
| Fatty acid metabolism | MAP1017c | 16 | 70.3 | 27.8 |
| MAP2698c | 20 | 73.9 | 31.4 | |
| MAP3190 | 16 | 75.8 | 33.3 | |
| Heat shock protein | MAP3268 | 16 | 58.9 | 16.4 |
| MAP3701c | 40 | 58.7 | 16.2 | |
| Hypothetical protein | MAP0593c | 40 | 57.3 | 14.8 |
| MAP3555 | 16 | 61.3 | 18.8 | |
| Phosphate metabolism | MAP0435c | 16 | 61.1 | 18.6 |
| Proteolysis | MAP2280c | 20 | 65.7 | 23.2 |
| Universal stress proteins | MAP1339 | 20 | 57.9 | 15.4 |
a
CT, cleavage time.
b
Fusion protein mass is the total mass of MBP (42.5 kDa) and the M. avium subsp. paratuberculosis protein.