Fig 1.

Histone H3 contains dual interaction interfaces for WDR5 and RBBP5. (A and B) GST pulldown assay with the indicated GST fusions and either in vitro translated Flag-tagged WDR5, Flag-RBBP5, or purified ASCOM complex with Flag-PA1. WDR5, RBBP5, and ASCOM were detected by immunoblotting with anti-Flag antibody. Coomassie blue staining of GST fusions is shown as a loading control. (C) Immobilized peptide pulldown assay using the indicated H3 peptides with either in vitro translated Flag-WDR5 or Flag-RBBP5. WDR5 and RBBP5 were detected by immunoblotting with anti-Flag antibody. All inputs are 5%. K4-me2, K4-dimethylated H3.