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. 2013 Dec;33(24):4936–4946. doi: 10.1128/MCB.00601-13

Fig 6.

Fig 6

Removal of H3K27 trimethylation is required for RA-induced transactivation. (A) Coimmunoprecipitation assays with the indicated antibodies using HEK293 cells transfected with scrambled siRNA (NC) or si-UTX. (B to D) ChIP showing levels of H3K27 trimethylation (B), H3K4 trimethylation (C), and RBBP5 occupancy (D) of the transcription start site (+1) and RARE regions of p21 in HEK293 cells transfected with scrambled siRNA (NC) or si-UTX. (E) Coimmunoprecipitation assays with the indicated antibodies using HEK293/Flag-PA1 cells transfected with si-UTX and either the wild-type (UTX-wt) or catalytically inactive (UTX-m) form of si-UTX-resistant UTX. (F) ChIP showing that RA-dependent recruitment of RBBP5 to the +1 region of p21 in HEK293 cells transfected with si-UTX is restored more readily by the wild-type UTX (UTX-wt) form than by the catalytically inactive (UTX-m) form of si-UTX-resistant UTX. In contrast, both forms of UTX were readily recruited to the +1 region of p21. WB, Western blotting. *, P < 0.05; **, P < 0.01.