Fig 5.
Mitochondrial tRNASerUGA and tRNATrpCCA contain i6A37, and the steady-state level of mt tRNASerUGA decreases in its absence. (A to N) PHA6 analysis of the six mt tRNAs that contain the A36A37A38 motif, using ACL or body probes, as indicated to the right of each panel. (O) Quantification of the PHA6 assay results in panels B to N using the formula noted in the text. The standard errors (bars) were determined from three independent experiments. (P) The ratio of mt tRNASerUGA or mt tRNATrpCCA to U5 is shown.