Fig 5.

Pathway array detections of post-LRP-1 signaling by eHsp90α. The parental HDFs (A) and LRP-1-knocked-down HDFs (B) were serum starved for 16 h and either not treated (−) or treated (+) with recombinant Hsp90α (10 μg/ml or 0.1 μM for 10 min). The cell extracts were subjected to protein kinase array screening (see Materials and Methods). Activated pathways include phospho-Stat5b, phospho-Stat5a, phospho-β-catenin, phospho-Akt (S437), and phospho-ERK1/2. Akt(T-308), however, was negative. (C and D) When the same blots shown in panels A and B were underexposed (1 min instead of 3 min), they also revealed the activation of ERK1/2 in parent HDFs (C, panel f versus panel e) but not in LRP-1-knocked-down HDFs (D, panel h versus panel g).