Fig 4.
CREB3L1 expression inhibits angiogenesis in tumors. (A to C) Immunohistochemistry for CD34 expression on blood vessels in tumors removed at day 40 from the footpad of rats injected with LN4D6 (A), LN4D6 vector control (B), and LN4D6 CREB3L1 (C) cells (1 × 106 cells). (D) A negative staining control assay was also performed on an LN4D6 vector section in which the primary antibody to CD34 was omitted. (E) The numbers of small vessels (<2 mm in diameter) and large vessels (>2 mm) in each field were qualified. A total of 9 fields from 3 different tumors in each group were assessed, and the results are expressed as vessels/field. The data are the means ± SE of the results of 2 independent experiments. **, P < 0.001.