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. 2013 Dec;33(24):4872–4888. doi: 10.1128/MCB.00711-13

Fig 2.

Fig 2

Growth curves of T. brucei bloodstream forms upon RNAi knockdown with 1 μg/ml of tetracycline (Tet). (a) AlaRS (Tb927.6.700); (b) ProRS (Tb927.10.12890); (c) LeuRS (Tb927.11.3730); (d) LysRS-1 (Tb927.8.1600). Data from panels a to d are the averages of at least three experiments ± SDs. (e) Quantitative RT-PCR analysis of T. brucei bloodstream-form RNA collected 24 h after tetracycline induction of RNAi from the cell lines for which the results are shown in panels a to d. Data were normalized with telomerase reverse transcriptase (TERT) relative to those for the noninduced matched controls and are the averages of three experiments ± SDs. RQ, relative quantification.