Table 1.
Plasmids used in this study with the GeneDB accession number and primers used for each gene cloned
| Primer no. | Plasmid used in this study | Gene or GeneDB accession no. of sequence cloned | Sequencea |
|
|---|---|---|---|---|
| Forward primer | Reverse primer | |||
| 1 | pLEW100-3v5 | 3v5 tag | CCCGGTCTCAAGCTTCTCGAGGGATCCGGTAAGCCTATCCCTAACCCTCTC | CCCGGTCTCAGATCTTTACTACGTGCTATCAAGACCGAGG |
| 2 | pLEW100-MetRS-3v5 | Tb927.10.1500 | CCCAAGCTTATGGCTCTAAAGCTGCTTTCAGA | CCCAGATCTTGTACTCTTTGTATTCTCTGTTGAGCG |
| 3 | pLEW100-ValRS-3v5 | Tb927.6.4480 | CCCGTCGACATGAAACAACTTGCTCCCCAG | CCCAGATCTGAGTAAACTCTTCATCTTTGTTAAACCCTC |
| 4 | pLEW100-SerRS-3v5 | Tb927.11.7170 | CCCGGTCTCAAGCTTCATATGGTGCTTGATATACAGCTGTTTC | CCCGGTCTCCTCGAGCTCCCCCTTGTCGGGT |
| 5 | pLEW100-GlyRS-3v5 | Tb927.11.9640 | CCCGGTCTCAAGCTTATGAGCACCGGGCTGC | CCCGGTCTCGGATCCCGTTGCGGCTGCCTG |
| 6 | pLEW100-CisRS-3v5 | Tb927.6.950 | CCCGGTCTCAAGCTTCATATGAAGGAAAGTGACGGTTTGTTAT | CCCGGTCTCCTCGAGTGATACTTCGGTGCTTTTTAAGCT |
| 7 | pLEW100-GluRS-3v5 | Tb927.6.4590 | CCCGGTCTCAAGCTTATGCTCCGGTTAGCTAACAAGTTTA | CCCGGTCTCGGATCCGACAGCTTGTTCTTTGTTCTGACTTT |
| 8 | pLEW100-MCP2-3v5 | Tb927.8.5330 | CCCGGTCTCAAGCTTATGGAAGGTCCTTTCCTCAGC | CCCGGTCTCGGATCCTGAAACGCTACCGTTGGG |
| 9 | pT7-IleRS-3v5 | Tb927.10.9190 | CACAAGCTCCTAGGAAGCTTCATGAATCGATGCTACTCC | CTCTAGGGATCCCTCGAGCGATTCACCAGCCGACGG |
| 10 | pT7-IleRS-3v5 ΔM1-G66 | Tb927.10.9190 | CACAAGCTCCTAGGAAGCTTCATGACTGGACCACTACAA | CTCTAGGGATCCCTCGAGCGATTCACCAGCCGACGG |
| 11 | pT7-GFP | GFP | CCCGGTCTCCTCGAGATGGTGAGCAAGGGCGAGGAGCTG | CCCGGTCTCGGATCCGGTACCCTACTTGTACAGCTCGTCCATGCC |
| 12 | pT7-IleRSM1-G66-GFP | Tb927.10.9190 | CCCGGTCTCAAGCTTATGAATCGATGCTACTCCCTCCTT | GCCCTTGCTCACCATCGTGGTTTTACTCGC (reverse #12.1) and CCCGGTCTCGGATCCCTACTTGTACAGCTCGTCCATGCC (reverse #12.2) |
| 13 | pT7-ProRS-3v5 | Tb927.10.12890 | CCCCGGTCTCAAGCTTATGCTGATGGCAACATGTATC | CCCCGGTCTCGGATCCATAGCTTCTCCCAAATAAAACCCA |
| 14 | PT7-ProRS-3v5 ΔM1-G46 | Tb927.10.12890 | CCCGGTCTCAAGCTTATGTCAGCAAGTAATTGT | CCCCGGTCTCGGATCCATAGCTTCTCCCAAATAAAACCCA |
| 15 | pLEW79-MH-TAP-MetRS | Tb927.10.1500 | CCCAAGCTTATGGCTCTAAAGCTGCTTTCAGA | CCCAGATCTTGTACTCTTTGTATTCTCTGTTGAGCG |
| 16 | pLEW79-MH-TAP-ProRS | Tb927.10.12890 | CCCGGTCTCAAGCTTATGTCAGCAAGTAATTGT | CCCCGGTCTCGGATCCATAGCTTCTCCCAAATAAAACCCA |
| 17 | pLEW79-MH-TAP-MCP2 | Tb927.8.5330 | CCCGGTCTCAAGCTTATGGAAGGTCCTTTCCTCAGC | CCCGGTCTCGGATCCTGAAACGCTACCGTTGGG |
| 18 | pLEW100-NTAP-IleRS | Tb927.10.9190 | CCCAAGCTTATGACTGGACCACTACAAAACTTTG | CCCAGATCTTTACGATTCACCAGCCGAC |
| 19 | pLEW100-NTAP | N-terminal TAP | ACTTCAATTACACCAAAAAGTAAAATTCACATGCACGATGAAGCCGT (forward #19.1) | CCCAGATCTTCATTAGGATCCCTCGAGAAGCTTAAGTGCCCCGGAGGAT (reverse #19.1) |
| ATAGCGGCCGCTATCGAT (forward #19.2) | GTGAATTTTACTTTTTGGTGTAATTGAAGT (reverse #19.2) | |||
| 20 | pET29-MCP2 | Tb927.8.5330 | CCCCATATGGAAGGTCCTTTCCTC | CCCCTCGAGTGAAACGCTACCGTTG |
| 21 | pQuadra-AlaRS | Tb927.6.700 | ATACCAATGTGATGGGGAGGCAATACTGTGGTCGT | ATACCATAGAGTTGGTGCGCCTTGCAGTATAGATG |
| 22 | pTrypRNAiGate-ProRS | Tb927.10.12890 | TTTCTTGATCACTATGCGGCTG | AAACCCTTAAGCTCCCAGTGGT |
| 23 | pTrypRNAiGate-LysRS | Tb927.8.1600 | ACGCATAATCCGGAGTTCAC | CTGAGCCGAAGGTCTTTACG |
| 24 | pTrypRNAiGate-LeuRS | Tb927.11.3730 | CGGAGGAGCTGTTGAAGAAC | CTCGGAGTAGGCACCAGAAG |
a
Restriction sites are shown underlined.