Table 5.
Unique peptide count and percent amino acid coverage of proteins in the MARS complex identified by TAP and mass spectrometry
| GeneDB accession no. or tag | GeneDB annotation | New name | Length (no. of amino acids) | Unique peptide count (% amino acid coverage)a |
|||||
|---|---|---|---|---|---|---|---|---|---|
| BF_MetRS-TAP | PF_MetRS-TAP | BF_MCP2-TAP | PF_MCP2-TAP | BF_IleRS-TAP | BF_ProRS-TAP | ||||
| Tb927.10.1500 | Methionyl-tRNA synthetase | Same | 773 | 53 (64.29) | 76 (81.11) | 2 (3.62) | 4 (6.99) | ||
| Tb927.10.12890 | Bifunctional aaRS | ProRS | 808 | 5 (10.15) | 73 (71.78) | ||||
| Tb927.9.5210 | Glutaminyl-tRNA synthetase | Same | 627 | 1 (3.19) | 36 (49.92 | 41 (54.55) | |||
| Tb927.7.2400 | Tyrosyl-tRNA synthetase | MCP1 | 425 | 6 (13.18) | 17 (44.00) | 2 (3.06) | |||
| Tb927.8.5330 | Tyrosyl/methionyl-tRNA synthetase | MCP2 | 223 | 2 (14.35) | 25 (87.44) | 20 (78.92) | |||
| Tb927.10.1250 | Hypothetical protein, conserved | MCP3 | 261 | 14 (61.69) | 17 (52.49) | ||||
| Tb927.10.9190 | Isoleucyl-tRNA synthetase | Same | 1143 | 3 (4.2) | |||||
| Tb927.11.9640 | Glycyl-tRNA synthetase | Same | 624 | 1 (1.92) | |||||
| Tb927.6.700 | Alanyl-tRNA synthetase | Same | 966 | 1 (2.28) | |||||
| Tb927.8.2240 | Tryptophanyl-tRNA synthetase | Same | 473 | 1 (1.90) | |||||
| Tb927.6.1880 | Aspartyl-tRNA synthetase | Same | 560 | 1 (2.68) | |||||
| CBP tagb | 26 | 2 (5.95) | 3 (7.03) | 1 (5.95) | 4 (8.1) | 2 (5.95) | |||
a
The data show the total unique peptide counts from two independent purification and mass spectrometry experiments for PF_MetRS-TAP, BF_ProRS-TAP, and BF_MCP2-TAP, three experiments for BF_MetRS-TAP, and one experiment for PF_MCP2-TAP and BF_IleRS-TAP. Peptides that were identified more than once in two or more experiments were only counted once. BF, bloodstream forms; PF, procyclic forms.
b
The CBP tag is part of the TAP tag and remains associated with the tagged protein after TEV protease digestion.