Table 6.
Steady-state kinetic parameters of MetRS and MetRS complex for l-methionine and tRNAMet
| Substrate and MetRS form | Km (mol/liter) | Vmax (nmol/min/μg) | kcat (s−1) | Efficiency [(mol/liter)−1 s−1] |
|---|---|---|---|---|
| l-Methionine | ||||
| MetRS complex | 7.0 × 10−5 ± 3.8 × 10−5 | 3.9 ± 0.3 | 10 ± 0.8 | 1.4 × 105 ± 2.0 × 104 |
| MetRS | 5.4 × 10−5 ± 4.2 × 10−5 | 2.4 ± 0.3 | 6.3 ± 0.7 | 1.2 × 105 ± 1.6 × 104 |
| tRNAMet | ||||
| MetRS complex | 1.0 × 10−7 ± 1.3 × 10−7 | 2.2 ± 0.3 | 5.8 ± 0.8 | 5.6 × 107 ± 6.0 × 106 |
| MetRS | 5.2 × 10−6 ± 2.9 × 10−6 | 2.4 ± 0.5 | 6.3 ± 1.4 | 1.2 × 106 ± 4.8 × 105 |