Fig 2.

Npl3 is required for translation. (A) npl3Δ100 is defective in general protein synthesis. The wild type, the translation initiation mutant prt1-1, and the indicated npl3 mutants were grown to log phase in medium lacking methionine. Strains were split and either retained at 25°C or shifted to 37°C for the indicated times before protein synthesis was measured by [³⁵S]methionine incorporation and quantified relative to the wild type (10 different experiments were quantified, and the standard deviations are shown). (B) The dimerization-defective npl3Δ100 mutant is hypersensitive to the translation inhibitor cycloheximide. Strains are shown on yeast extract-peptone-dextrose (YPD) plates and YPD plates containing 0.04 μg of cycloheximide/ml upon incubation at 25°C for 3 days.