Fig 1.

A genetic selection to monitor ploidy reduction in C. tropicalis. Diploid a/a (CAY2059 and CAY2060) and α/α (CAY3371 and CAY3372) C. tropicalis cells were mated to generate tetraploid strains (CAY3373, CAY3374, and CAY3375) that were heterozygous at the GAL1, MTL, HIS1, and ARG4 loci. Loss of GAL1 function was screened for by selection on 2-deoxygalactose (2-DOG) medium, which selects for Δgal1 cells. Chromosomal positions of genes are implicated based on chromosome positioning in the related species C. albicans. Linkage of MTL and HIS1 loci in C. tropicalis was also demonstrated in the present studies.