Fig 4.

C. tropicalis parasexual progeny are mating competent. (A and B) Representative images of mating projections (A) and zygotes (B) seen during mating of parasexual progeny with wild-type strains. (C) Characterization of MTL loci from four parasexual remating products (parasexual strains [PS] C6 to C9), compared with the parasexual parent (B2) and MTLa parental strain (CAY2060). (D to G) Cell cytometric data showing ploidy of parasexual remating products PS C6 (D), PS C7 (E), PS C8 (F), and PS C9 (G). The x axis (Sytox) represents a linear scale of fluorescence, and the y axis (counts) represents a linear scale of cell number. Diploid (light gray) and tetraploid (dark gray) controls are shown in the background as described in the legend of Fig. 3.