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. 2013 Sep;20(9):1370–1376. doi: 10.1128/CVI.00140-13

Fig 1.

Fig 1

(A) SDS-PAGE (12%) of the seven recombinant proteins purified by affinity chromatography. (B) Western blot analysis of the seven recombinant proteins subjected to 12% SDS-PAGE, electrotransferred to a nitrocellulose membrane, and probed with mouse monoclonal anti-histidine-tagged antibody (diluted 1:10,000) and peroxidase-conjugated anti-mouse IgG (diluted 1:4,000) as the secondary antibody. Lane 1, MHP0107 (38 kDa); lane 2, MHP0272 (59 kDa); lane 3, MHP0418 (37 kDa); lane 4, MHP0443 (54 kDa); lane 5, MHP0372 (44 kDa); lane 6, MHP0660 (48.5 kDa); lane 7, MHP0487 (33 kDa). Arrows indicate the bands corresponding to the recombinant proteins.