Table 1.
Real-time quantitative RT-PCR probes and primers for proinflammatory cytokines, inflammatory chemokines, and type I and II interferons
| RNA target | Sequence typeb | Probe/primer sequencec | Accession no.a |
|---|---|---|---|
| 28S | Probe | 5′-(FAM)-AGGACCGCTACGGACCTCCACCA-(TAMRA)-3′ | X59733 |
| F | 5′-GGCGAAGCCAGAGGAAACT-3′ | ||
| R | 5′-GACGACCGATTGCACGTC-3′ | ||
| IL-1β | Probe | 5′-(FAM)-CCACACTGCAGCTGGAGGAAGCC-(TAMRA)-3′ | AJ245728 |
| F | 5′-GCTCTACATGTCGTGTGTGATGAG-3′ | ||
| R | 5′-TGTCGATGTCCCGCATGA-3′ | ||
| IL-6 | Probe | 5′-(FAM)-AGGAGAAATGCCTGACGAAGCTCTCCA-TAMRA)-3′ | AJ250838 |
| F | 5′-GCTCGCCGGCTTCGA-3′ | ||
| R | 5′-GGTAGGTCTGAAAGGCGAACAG-3′ | ||
| IL-15 | Probe | 5′-(FAM)-CCACCCAATCCAGGAAATGTTAACCCA-(TAMRA)-3′ | NM_204571.1 |
| F | 5′-AGCTGAACTGCTGCCACATTT-3′ | ||
| R | 5′-TTTCCTCTGTTCTTCTTTGTCTGAATC-3′ | ||
| IL-18 | Probe | 5′-(FAM)-CCGCGCCTTCAGCAGGGATG-(TAMRA)-3′ | AJ416937 |
| F | 5′-AGGTGAAATCTGGCAGTGGAAT-3′ | ||
| R | 5′-ACCTGGACGCTGAATGCAA-3′ | ||
| IFN-γ | Probe | 5′-(FAM)-TGGCCAAGCTCCCGATGAACGA-(TAMRA)-3′ | YO7922 |
| F | 5′-GTGAAGAAGGTGAAAGATATATCATGGA-3′ | ||
| R | 5′-GCTTTGCGTGGATTCTCA-3′ | ||
| IFN-α | Probe | 5′-(FAM)-CTCAACCGGATCCACCGCTACACG-(TAMRA)-3′ | U07868 |
| F | 5′-GACAGCCAACGCCAAAGC-3′ | ||
| R | 5′-GTCGCTGCTGTCCAAGCATT-3′ | ||
| CXCLi1 | Probe | 5′-(FAM)-CCACATTCTTGCAGTGAGGTCCGCT-(TAMRA)-3′ | AF277660 |
| F | 5′-CCAGTGCATAGAGACTCATTCCAAA-3′ | ||
| R | 5′-TGCCCATCTTTCAGAGTAGCTATGAACT-3′ | ||
| CXCLi2 | Probe | 5′-(FAM)-CTTTACCAGCGCGTCCTACCTTGCGACA-(TAMRA)-3′ | AJ009800 |
| F | 5′-GCCCTCCTCCTGGTTTCAG-3′ | ||
| R | 5′-TGGCACCGCCAGCTCATT-3′ |
a
For the genomic DNA sequence.
b
F, forward; R, reverse.
c
FAM, 5-carboxyfluorescein; TAMRA, N,N,N,N′-tetramethyl-6-carboxyrhodamine.