Fig 8.

Cps2E glycosyltransferase activities in recombinant proteins expressed in E. coli. (A) Glycosyltransferase assays were performed as for Fig. 6A. Samples were assayed using 0.5 and 2 μg of total membrane protein, which was in the linear range for the Cps2E⁺ strain (KJ4152). Values are the means ± standard errors of the two concentrations, normalized to cpm/μg. (B) Cps2E protein in membranes used for glycosyltransferase assays for panel A. Experiments were performed as described for Fig. 6B, loading 5 μg of total membrane protein. Values are the means of two immunoblots, normalized to the Cps2E⁺ strain. Strains: Cps2E⁻, RC124; Cps2E⁺, KJ4152; H258R, DJ252; A162S, DJ253; D167Y, DJ254; L200F, DJ269: and V196G, DJ270.