Fig 2.

Positively selected genes implicated in conferral of different host cell phenotypes. (A) Representative confocal images of untransfected HeLa cells or cells transfected with empty vector (GFP only) or BPSS0945 (GFP tagged). Cells were fixed, permeabilized, and stained for nuclei (DAPI; blue) and actin (Phalloidin; red). Untransfected cells or cells transfected with empty vector (rows I and II) did not exhibit any host cell phenotypes. Cells transfected with BPSS0945 exhibited multinucleate giant cell formation (row III). Bars, 10 μm. (B) Quantitative analysis of multinucleate giant cell formation in untransfected cells or cells transfected with empty vector or BPSS0945. Values on the y axis are presented as percentages of the total number of transfected cells (n = 100), obtained from three independent experiments. The asterisk indicates a P value of <0.05 relative to untransfected or empty vector-transfected cells. (C) Cells transfected with BPSL2198 exhibited cell death (row III), in contrast to untransfected cells (row I) or empty vector-transfected cells (row II). Bars, 10 μm. (D) Quantitative analysis of cell death induction in untransfected cells or cells transfected with empty vector or BPSL2198. Values are presented as percentages of the total number of transfected cells (n = 100), obtained from three independent experiments. The asterisk indicates a P value of <0.05 relative to untransfected or empty vector-transfected cells.