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Importance of rsiR in human TNF suppression by L. reuteri. The rsiR mutant could not suppress TNF production by activated THP-1 cells. Genetic inactivation of rsiR resulted in a complete loss of TNF suppression in THP-1 cells, which was not fully complemented in the presence of supplemental histidine. ***, P = 0.0001 using an unpaired t test comparing wild-type strain 6475 and the 6475 ΔrsiR mutant.
