Fig 5.

Role of rsiR in suppression of intestinal inflammation in vivo by L. reuteri. The L. reuteri 6475::rsiR mutant conferred a diminished protective effect in a TNBS-induced colitis mouse model compared to that conferred by wild-type strain 6475. Complementation of the L. reuteri 6475::rsiR mutant (6475::rsiR/pJKS104) restored the anti-inflammatory and protective effects. (A) Macroscopic evaluation of colitis was performed using Wallace scoring criteria. Data are presented using scatter dot plots. Statistical significance among all groups was determined at a P value of <0.0001 by the nonparametric Kruskal-Wallis test. Statistical significance between two groups was assessed by the Mann-Whitney U test followed by a Bonferroni adjustment. (B) Plasma concentrations of SAA were measured by ELISA. Statistical significance among all groups was determined as described for the Wallace score for panel A. Differences between experimental groups are shown as the mean concentration ± SEM. ****, P ≤ 0.0001; ***, P < 0.001; **, P < 0.005; *, P < 0.05.