Fig 3.

(A) Mannan binding by different MrkD variants in a growth assay. White bars denote MrkD protein variants located within the primary zone. Gray bars denote MrkD protein variants located in the external zone. The statistical differences labeled in the figure are based on a comparison with the evolutionarily oldest protein variant, C3091. The mean values of three experiments are shown along with standard errors of the means. P values were calculated by using Student's t test. (B) Biofilm formation by different MrkD variants. White bars denote MrkD protein variants located within the primary zone. Gray bars denote MrkD protein variants located in the external zone. The statistical differences labeled in the figure are based on comparisons with the evolutionarily oldest protein variant, C3091. The mean values of three experiments are shown, along with standard errors of the means. P values were calculated by using Student's t test.