Fig 7.

Effect of 2-ketobutyrate, 2-aminobutyrate, and pantothenate on l-Val productivity. The HPLC chromatograms generated by amino acid analysis of the stationary-phase culture supernatant of WT (left) and ΔyggS mutant (right) strains cultivated alone (A and E) or in the presence of 1 mM 2-ketobutyrate (B and F), 1 mM 2-aminobutyrate (C and G), and 1 mM pantothenate (D and H). The cells were grown in the M9-glucose medium at 30°C. Symbols: *, 2-aminobutyrate; V, valine; I, isoleucine; #, norleucine.