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The PA103 exsA::Ω strain carrying the indicated PexoT-lacZ transcriptional reporters was transformed with AxsA (pAxsA), PxsA (pPxsA), or VxsA (pVxsA) expression vectors. The resulting strains were cultured in the presence of EGTA and assayed for β-galactosidase activity. Activation by pAxsA (open bars), pPxsA (hatched bars), and pVxsA (gray bars) is reported as the percent activity of the mutant promoters normalized to the activity of the wild-type PexoT promoter.
