Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The CTnDOT mob genes are transcriptionally linked. To address whether the mob genes form a polycistronic transcript, RT-PCR was performed using primers that amplified the regions of the mobA-B and mobB-C intergenic junctions from BT4001 pLYL72 pGW45 induced with tetracycline (1 μg/ml). Amplicons for both of these primer sets were observed, thus indicating that the mob genes are transcriptionally linked and organized as an operon.
