Fig 4.
More mob transcript is detectable from CTnDOT than from CTnERL. (A) Qualitative RT-PCR was performed using primers to detect mobA from a strain containing CTnERL (BT4104) and CTnDOT (BT4007) both with and without Tc induction. No transcript is detectable in the absence of Tc, and yet, upon exposure to Tc, a mob transcript is detected. However, more mobA transcript is seen in cells containing CTnDOT than in cells containing CTnERL. rpoD is a reference marker used to ensure equivalent RNA concentrations in samples. (B) RT-qPCR was performed using primers to detect mobB. RNA content was normalized to the reference marker rpoD, and the ΔΔCT was calculated comparing induced (+) to noninduced (− Tc) levels.