Table 2.
Primer function and name | 5′ to 3′ sequence |
---|---|
Cloning | |
GRW54 fwd | GATGACTGCAGGGAATCACAAT |
GRW54 rev | TCTTGATAAGAGCTCTAGAAGTCATT |
GRW55 fwd | TGAAAGGTATCTGCAGTCCGT |
GRW55 rev | GGATCGAGCTCGGGATTTCT |
RT-PCR | |
mobA fwd | TCAAGGTGCTGAAGGTGGACAAGA |
mobA rev | TCTGACGGCTCAGTTTCACGAGTT |
mobB fwd | TTTCCGTGCTGCAAGGCACTTATG |
mobB rev | ATGAAAGCCTCTTCCTCGGCATCT |
mobC fwd | TGAACCGCCGCTACGAGGATATTT |
mobC rev | TCGGGATTGTTGATGTCCAGCGTA |
mobA-B fwd | TGGACTACTACACCAAGCTATCGG |
mobA-B rev | TTTCCAGATACTCGCGGGC |
mobB-C fwd | ACAACCGCCGATACCGTTATCCAT |
mobB-C rev | CCCTTTCGTACCCAGACAGGACA |
RpoD 67 fwd | TTTAATCTGACGAACGCGCTCACG |
RpoD 548 rev | ACGCTGTATGGTGGATTCGTCAGT |
RT-qPCR | |
mobA fwd | ACAACCGTTTCCTCGCCATGTTTG |
mobA rev | TCTTGTCCACCTTCAGCACCTTGA |
mobA-B fwd | AACTCGTGAAACTGAGCCGTCAGA |
mobA-B rev | TGTAGATGCGGTTGGTGGTGAGAA |
mobB fwd | ACAACCGCCGATACCGTTATCCAT |
mobB rev | ATGAAAGCCTCTTCCTCGGCATCT |
RpoD 467 fwd | ACCTGATTCAACGGGAGACGACA |
RpoD 548 rev | ACGCTGTATGGTGGATTCGTCAGT |