Skip to main content
. 2013 Dec;195(24):5431–5438. doi: 10.1128/JB.00691-13

Table 2.

Primers used in this study

Primer function and name 5′ to 3′ sequence
Cloning
    GRW54 fwd GATGACTGCAGGGAATCACAAT
    GRW54 rev TCTTGATAAGAGCTCTAGAAGTCATT
    GRW55 fwd TGAAAGGTATCTGCAGTCCGT
    GRW55 rev GGATCGAGCTCGGGATTTCT
RT-PCR
    mobA fwd TCAAGGTGCTGAAGGTGGACAAGA
    mobA rev TCTGACGGCTCAGTTTCACGAGTT
    mobB fwd TTTCCGTGCTGCAAGGCACTTATG
    mobB rev ATGAAAGCCTCTTCCTCGGCATCT
    mobC fwd TGAACCGCCGCTACGAGGATATTT
    mobC rev TCGGGATTGTTGATGTCCAGCGTA
    mobA-B fwd TGGACTACTACACCAAGCTATCGG
    mobA-B rev TTTCCAGATACTCGCGGGC
    mobB-C fwd ACAACCGCCGATACCGTTATCCAT
    mobB-C rev CCCTTTCGTACCCAGACAGGACA
    RpoD 67 fwd TTTAATCTGACGAACGCGCTCACG
    RpoD 548 rev ACGCTGTATGGTGGATTCGTCAGT
RT-qPCR
    mobA fwd ACAACCGTTTCCTCGCCATGTTTG
    mobA rev TCTTGTCCACCTTCAGCACCTTGA
    mobA-B fwd AACTCGTGAAACTGAGCCGTCAGA
    mobA-B rev TGTAGATGCGGTTGGTGGTGAGAA
    mobB fwd ACAACCGCCGATACCGTTATCCAT
    mobB rev ATGAAAGCCTCTTCCTCGGCATCT
    RpoD 467 fwd ACCTGATTCAACGGGAGACGACA
    RpoD 548 rev ACGCTGTATGGTGGATTCGTCAGT