Table 1.
Plasmids and strains used in this study
| Plasmid or strain | Description | Reference |
|---|---|---|
| Plasmid | ||
| pDSM430 | pET21A:: fur | 10 |
| H. pylori strains | ||
| G27 | WT H. pylori | 51 |
| DSM300 | G27 Δfur::cat; Cmr | 25 |
| E. coli strains | ||
| DSM431 | BL21(DE3) Rosetta/pLys Δfur (pDSM430); Ampr Cmr Kanr | 10 |
| DSM1215 | Top10 (pGEM-T Easy::HP0653 promoter region AAATGA apo-Fur box I ACACAC); Ampr | This study |
| DSM1384 | Top10 (pGEM-T Easy::HP0653 promoter region AAATGA apo-Fur box II ACACAC); Ampr | This study |
| DSM1385 | Top10 (pGEM-T Easy::HP0653 promoter region AAATGA apo-Fur boxes I and II ACACAC); Ampr | This study |
| DSM1386 | Top10 (pGEM-T Easy::HP0653 promoter region AAATGA apo-Fur box III ACACAC); Ampr | This study |
| DSM1387 | Top10 (pGEM-T Easy::HP0653 promoter region AAATGA apo-Fur boxes I, II, and III ACACAC); Ampr | This study |