Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| P. aeruginosa strains | ||
| PAO1 | Wild-type lab stock | 90 |
| PSL317 | ΔalgR | 51 |
| PAO1 algRD54E | algR12 (or algRD54E), encoding AlgR D54E | This study |
| WFPA8 | algR7 (or algRD54N), encoding AlgR D54N | 25 |
| PAZ-1 | ΔalgZ | 26 |
| PAZ algRD54E | ΔalgZ algR12 | This study |
| PAO1A | pilA::Tc | Daniel Hassett |
| PAO1C | fliC::Gm | Daniel Hassett |
| PAO1 ΔrhlA | ΔrhlA | Michael Vasil |
| PDO111 | rhlR::Tn501 | 91 |
| PAO1 rhlA::lacZ | rhlA::lacZ (in attB site) | This study |
| PSL317 rhlA::lacZ | ΔalgR rhlA::lacZ (in attB site) | This study |
| PAO1 algRD54E rhlA::lacZ | algR12 rhlA::lacZ (in attB site) | This study |
| WFPA8 rhlA::lacZ | algR7 rhlA::lacZ (in attB site) | This study |
| PAZ rhlA::lacZ | ΔalgZ rhlA::lacZ (in attB site) | This study |
| PDO111 rhlA::lacZ | rhlR::Tn501 rhlA::lacZ (in attB site) | This study |
| PAO1 rhlI::lacZ | rhlI::lacZ (in attB site) | 28 |
| PSL317 rhlI::lacZ | ΔalgR rhlI::lacZ (in attB site) | 28 |
| PAO1 algRD54E rhlI::lacZ | algR12 rhlI::lacZ (in attB site) | This study |
| PAO1 algRD54N rhlI::lacZ | algR7 rhlI::lacZ (in attB site) | This study |
| E. coli strains | ||
| DH5α | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ− thi-1 gyrA96 relA1 | Lab strain |
| Sm10 | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu | Lab strain |
| BL21 Rosetta | F− ompT hsdSB(rB− mB−) gal dcm pRARE2 (Cmr) | Novagen |
| Plasmids | ||
| pGEM-T Easy | PCR product cloning vector | Promega |
| pEX18Gm | Suicide cloning vector; Gmr | 31 |
| pEX18Gm algRKO | algR deletion vector; Gmr | This study |
| pEX18Gm algRD54E | algRD54E allelic replacement vector; Gmr | This study |
| pEX18Gm algRD54N | algRD54N allelic replacement vector; Gmr | This study |
| pHERD30T | pBAD-based Pseudomonas expression vector; Gmr | 30 |
| pHERD30T algR | algR in pHERD30T; Gmr | This study |
| pHERD30T algRD54N | algRD54N in pHERD30T; Gmr | This study |
| pHERD30T algRD54E | algRD54E in pHERD30T; Gmr | This study |
| pHERD30T rhlAB | rhlAB in pHERD30T; Gmr | This study |
| pGEX4T-3 | GST fusion expression vector; Ampr | GE Healthcare Life Sciences |
| pGEX4T-3 algR | algR in pGEX4T-3; Ampr | This study |
| pGEX4T-3 algRD54N | algRD54N in pGEX4T-3; Ampr | This study |
| pGEX4T-3 algRD54E | algRD54E in pGEX4T-3; Ampr | This study |
| Mini-CTX-1 | attB site chromosomal integration vector | 92 |
| Mini-CTX-1 lacZ | attB site chromosomal integration vector with promoterless lacZ | This study |
| CTX-1 fimU::lacZ | fimU promoter cloned into mini-CTX-1 lacZ | This study |
| CTX-1 rhlA::lacZ | rhlA promoter cloned into mini-CTX-1 lacZ | This study |
| pFLP2 | Source of FLP recombinase; Ampr | 31 |
| pRK2013 | Tra+ Mob+ ColE1 replicon; Kmr | 93 |