Fig 2.

ESAT-6 and CFP-10 secretion and expression in M. tuberculosis strains producing EspA variants. Immunoblots of culture filtrates (CF) at 10 μg/well and cell lysates (CL) at 5 μg/well of the espA::Tn+pMD31 control strain, the espA::Tn+pMDespACD strain expressing EspA^WT and strains expressing EspA^F50R, EspA^W55R, EspA^G57R, and EspA^K62A (A) and the espA::Tn+pMD31 control strain, the espA::Tn+pMDespACD strain expressing EspA^WT, and strains expressing EspA^F5R, EspA^I23R, EspA^K41A, and EspA^W55R (B) grown in Sauton's medium without Tween 80 for 6 days. Antibodies used are indicated, and the EspA band is indicated by white arrows. Results are representative of 2 independent experiments.