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. Author manuscript; available in PMC: 2014 Dec 2.
Published in final edited form as: Curr Biol. 2013 Nov 7;23(23):2365–2374. doi: 10.1016/j.cub.2013.10.011

Figure 4.

Figure 4

KaiA promotes expression and activity of endogenous Hox hydrogenase (H2ase).

(A) kaiA-enhanced mRNA microarray profiles of all H2ase subunits and H2ase maturation genes (as denoted) in the kaiA-overexpressing strain with (red) or without IPTG (blue).

(B) Hydrogenase activity increases in the {ATG}kaiA-OX strain relative to the wild-type (WT) strain in the absence or presence of IPTG induction. Data are represented as mean +/− SD from three independent experiments and analyzed with t-test. Note that the {ATG}kaiA overexpression construct is slightly “leaky”, and even at 0 μM IPTG, there is some extra expression of KaiA above that in WT.

(C) KaiA causes constantly high levels of hydrogenase activities under continuous light conditions. Following a 12h of dark pulse, the endogenous hydrogenase activities were measured from samples of the WT and {ATG}kaiA-OX strains at the indicated times in LL.